-I(IN VITRO) DNA SYNTHESIS REACTIONS USING PHI 29 DNA POLYMERASE AND A DNA FRAGMENT ENCODING SAID POLYMERASE

摘要

For determining an improved method of the nucleotide base sequence of a DNA molecule. Method includes the DNA molecule that annealing has a primer molecule that can hybridize to DNA molecule; It incubates in a container, include four different deoxynucleoside triphosphates, one DNA polymerases, and the mixture of the annealing in agency is terminated in one or more DNA synthesis that a specific nucleotide base terminates DNA synthesis, wherein each agency terminates DNA synthesis in a different nucleotide base; The DNA products for separating the reaction incubated according to size, thus may determine that at least part of the nucleotide base sequence of DNA. Improvement be once a& phgr archaeal dna polymerase regulation; 29 type DNA polymerases.