DNA synthesis reactions (in vitro) employing DNA polymerase modified phi 29 and a DNA fragment encoding said polymerase.

摘要

IMPROVED METHOD FOR DETERMINING A SEQUENCE OF BASE Nucleotide A DNA molecule. The method includes STRENGTHENING DNA molecule with a molecule of hybridizing CAPABLE PRIMER ON DNA molecule; Incubating MIXTURE ENHANCED IN A CONTAINER containing four triphosphates deoxynucleoside DIFFERENT, a DNA polymerase and one or more terminating agents DNA synthesis ENDING DNA synthesis ON AN Nucleotide SPECIFIED, WHERE EACH OF AGENTS ENDING THE DNA synthesis in a nucleotide base other; And separating DNA products INCUBATION REACTION BY SIZE, YOU CAN DETERMINE THUS AT LEAST ONE PART OF BASE SEQUENCE OF DNA nucleotide. THE IMPROVEMENT IS MADE OF SUPPLY DNA polymerase IS A TYPE phi29 DNA polymerase.