IMMOBILIZATION OF ENZYMES BY CROSS-LINKING WITH A CROSS-LINKING AGENT AND A POLYMER CONTAINING 1-AMINO ETHYLENE MOIETIES

摘要

PCT No. PCT/DK90/00305 Sec. 371 Date Feb. 28, 1992 Sec. 102(e) Date Feb. 28, 1992 PCT Filed Nov. 23, 1990 PCT Pub. No. WO91/08287 PCT Pub. Date Jun. 13, 1991.Physically strong particles containing an immobilized enzyme are prepared for use in a fixed bed-column. The particles are prepared by adding a homopolymer of 1-amino ethylene or a copolymer of 1-amino ethylene and N-vinyl formamide to an aqueous medium containing an enzyme, adding a cross-linking agent to cross-link the enzyme and polymer and cause flocculation, and dewatering, sub-dividing and drying the resultant flocculant. Preferably, the enzyme is glucose isomerase and the cross-linking agent is glutaraldehyde, polyazetidine or diisocyanate.