METHOD FOR PRODUCTION OF COMPLEX FERMENT PREPARATION WHICH CONTAINS amp;beta;-1,3-GLUCANASE AND CHITINASE

摘要

FIELD: biotechnology, microbiological industry. SUBSTANCE: desired products are isolated of fodder antibiotics. The process is carried out with the help of water or by 0.05-0.1 M buffer solution of sodium acetate at 15-25 C and pH 5.5 within 30-120 min. Thus prepared extract is collected; b-1,3-glucanase and chitinase being containing in it are purified. Said purification is carried out by precipitation with ammonia sulfate or by concentration which takes place by ultracentrifugating or by chromatography. EFFECT: decreases duration of the process, decreases number of its stages. 3 cl, 1 dwg