A method for the derivatization of amino acids with phenyl isothiocyanate and devices for this purpose for the hplc

摘要

The derivatisation of amino acids using phenyl isothiocyanates for HPLC analysis can be greatly simplified by the removal of excess phenyl isothiocyanate after addition of a suitable aqueous solution for phase separation and removal of the phenyl isothiocyanate-containing lipophilic phase by filtration through a suitable membrane filter or adsorption of the lipophilic phase on a suitable material. The previously customary complicated vacuum drying of the amino acid derivatives is thus unnecessary; the derivatisation of amino acids with phenyl isothiocyanates can now be readily automated.