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Method for the preparation of stable cell lines from transgenic animals for the preparation of certain proteins, tumor cell lines and the resulting proteins.
Method for the preparation of stable cell lines from transgenic animals for the preparation of certain proteins, tumor cell lines and the resulting proteins.
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机译:从转基因动物制备稳定细胞系的方法,用于制备某些蛋白质,肿瘤细胞系和所得蛋白质。
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摘要
The invention relates to a process for the preparation of a cell line which is stable for the production of a protein which is determined by the induction of tumours in transgenic animals and the selection of the descending who develop tumours. In a variant, it is introduced in the transgenic animals, the sequence encoding the protein, it selects the daughter products which have integrated the sequence, and are merged the lymphocytes of the rate of these animals with myeloma cells. In the two variants, the cell lines derived from tumors produce proteins, in particular of therapeutic interest.
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