One-pot synthesis of oligosaccharides using a CMP- sialic acid regenerating system and multi-enzyme

摘要

(57) a single reaction vessel method for the synthesis of [summary] sialylated galactoside is disclosed. Generates a galactosyl glycoside novel using catalyzes the reaction of the acceptor and galactose-containing substrate β- galactosidase, and sialylated it then used a multi-enzyme synthesis system of the cycle and its synthesis the CMP- sialic acid generates and sialylated galactosyl glycoside that this is produced in the presence of α- sialyltransferase. The value of K _m / V _max on galactosyl glycoside that has been generated as a substrate for α- sialyltransferase is less than one-third of K _m / V _max values ​​for the galactose-containing substrates α- sialyltransferase.