DETECTING PROBE FOR ANTI DOUBLE-STRANDED DNA ANTIBODY IN HUMOR SUCH AS SLE SERUM

摘要

PROBLEM TO BE SOLVED: To reduce measuring errors and enable correct measurements by labelling a linear dsDNA formed by cutting by means of a restriction enzyme, at its terminal. ;SOLUTION: A DNA tracer obtained by labelling a terminal of a linear dsDNA obtained by cutting of a cyclic dsDNA such as plasmid DNA or the like by means of a restriction enzyme is used. As a living body including the cyclic dsDNA such as plasmid DNA or the like, germs such as colon bacilli, Bacillus subtilis, etc., or yeast such as Saccharomyces-cereviciae or the like are utilizable. When the dsDNA is labelled only at its terminal, DNA molecules are never damaged by nicks, etc., and therefore the dsDNA can be labelled in the most stable state. As a labelling substance, deoxyribonucleotide phosphate labelled with radiactive substance, enzyme, fluorescent substance or chemical emission substance, or the like is used.;COPYRIGHT: (C)1996,JPO