NUCLEIC ACID PREPARATION FOR IMMUNIZATION AND IMMUNIZING METHOD USING THE ACID

摘要

PURPOSE: To obtain the subject preparation consisting of a linearized DNA having a gene coding a pathogen-originated immunogen and connected to the downstream side of a gene-manifestation promoter functioning in an animal cell and exhibiting excellent effect on imparting immunity against the pathogen. CONSTITUTION: This nucleic acid preparation for immunization consists of a linearized DNA and exhibits excellent immunizing effect in the immunization against various pathogens by the DNA transduction into an animal. The preparation is produced by using e.g. a plasmid having an avian -actin gene promoter as the promoter for the manifestation of gene functioning in an animal cell, incising the plasmid at the HindIII site positioned at the 3'-side (downstream) of the promoter, recovering the fragment containing the promoter from the gel by electrophoresis, subjecting the fragment to blunt end treatment, dephosphorylating the product and connecting the dephosphorylated site with a gene coding an immunogen originated from a pathogen such as avian Newcastle disease virus(NDV)-F protein.