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CYTOPLASMIC EXPRESSION OF ANTIBODY IN ESCHERICHIA COLI, ANTIBODY FRAGMENT AND ANTIBODY FRAGMENT FUSION MOLECULE
CYTOPLASMIC EXPRESSION OF ANTIBODY IN ESCHERICHIA COLI, ANTIBODY FRAGMENT AND ANTIBODY FRAGMENT FUSION MOLECULE
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机译:大肠杆菌中抗体的细胞膜表达,抗体片段和抗体片段融合分子
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摘要
Prodn. of recombinant antibodies (Ab), Ab fragments or Ab fragment/enzyme fusion proteins, where the enzyme is a cytoplasmic mammalian or E. coli enzyme, is effected using a thioredoxinreductase deficient E. coli strain, the expression prods. being isolated from the cytoplasm. Also claimed are: (1) Ab fragment/enzyme fusion protein in which the enzyme is E. coli beta -glucuronidase; and (2) gene encoding the fusion protein of (1).
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