FIBRINOLYTIC PROTEIN HAVING BLOOD COAGULATION INHIBITING PROPERTY

摘要

New peptides (I) with fibrinolytic and anticoagulant activity comprise a plasminogen-activating amino acid (aa) sequence (A) fused at the N and/or C terminus to a thrombin and/or factor Xa inhibiting aa sequence (B). Excluded are (I) where (A) is Ser47 to Leu411 sequence of unglycosylated urokinase linked at the C-terminus to sequences (i), (ii) or (iii): T1-RP-T2-GGGGNGDFEEIPEEYL-T3 (i); T1-RPFLLRNPNDKYEPFWEDEEDKNE (ii); T1-RPSSEFEEFEIDEEEK (iii); T1 = P or V; T2 = L or bond; T3 = Q or OH. Also new are plasmids for producing (I) in E. coli, contg. an operon consisting of a regulatable promoter; Shine-Dalgarno (ribosome binding) sequence; start codon; synthetic gene for (I); and 1 or 2 downstream terminators.