RICE NADH-DEPENDENT REDUCTASE GENE

摘要

PURPOSE: To obtain a new gene having a specific base sequence, coding for a rice NADH-dependent reductase, capable of giving resistance to pathogenic bacteria to a plant such as rice through linkage to a manifestation promoter system for plant cells and transfection into said plant. ;CONSTITUTION: This new gene has a base sequence containing a base sequence expressed by the formula, coding for a rice NADH-dependent reductase, capable of controlling toxins produced by phytopathogenic bacteria and the peripheral metabolites by its linking to a promoter manifestative in plant and its transfecting into a plant to effect manifestation, thus enabling plants such as rice to be furnished with resistance to pathogenic bacteria. This new gene is obtained by extracting a mRNA from rice anther by a conventional method, synthesizing a cDNA using this mRNA to make a cDNA library which is then screened with a probe followed by recovering the aimed gene from a positive clone.;COPYRIGHT: (C)1996,JPO