PURPOSE: To provide a method for determination/activity measurement of a very small amount of proteinase A, for example, in beer and to obtain a substrate useful for the method. ;CONSTITUTION: An octapeptide derivative of the formula PG-A3-A4-A5-A5-Phe- Phe-Arg-Leu-FlG [PG shows an amino group-protecting group; A3, A4, A5 and A6 are each an amino acid shown by Ala, Asp, Lys, Leu, Arg, Ser or Pro; FlG shows a fluorescent group such as 4-methylcoumaryl-7-amide (MCA)]. This method for measuring proteinase A activity and foam stability of beer uses a nanapeptide derivative of the formula fg-A3-A4-A5-A6-Phe-Phe-Arg-Leu-Lys(Dnp)-NH2 [fg is a fluorescent group of (7-methoxycoumarin-4-yl)acetyl (MOCAc) group or anthraniloylbenzyl (ABz) group; A3, A4, A5 and A6 are each independently an amino acid shown by Ala, Asp, Lys, Leu, Arg, Ser or Pro; (Dnp) is dinitrophenyl group].;COPYRIGHT: (C)1996,JPO
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机译：目的：提供一种用于测定/测量啤酒中极少量蛋白酶A的方法，并获得可用于该方法的底物。 ;组成：式PG-A 3 -A 4 -A 5 -A 5 的八肽衍生物-Phe-Phe-Arg-Leu-FlG [PG表示氨基保护基; A 3 ，A 4 ，A 5 和A 6 是Ala，Asp， Lys，Leu，Arg，Ser或Pro;图1G显示了荧光基团，例如4-甲基香豆基-7-酰胺（MCA）。这种测量啤酒蛋白酶A活性和泡沫稳定性的方法是使用分子式为fg-A 3 -A 4 -A 5 的纳肽衍生物-A 6 -Phe-Phe-Arg-Leu-Lys（Dnp）-NH 2 [fg是（7-甲氧基香豆素-4-基）乙酰基的荧光基团（MOCAc）基团或蒽基苄基（ABz）基团; A 3 ，A 4 ，A 5 和A 6 分别独立地是Ala，Asp所示的氨基酸，Lys，Leu，Arg，Ser或Pro; （Dnp）是二硝基苯基] 。;版权：（C）1996，日本特许厅
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