PURPOSE: To provide a method for determination/activity measurement of a very small amount of proteinase A, for example, in beer and to obtain a substrate useful for the method. ;CONSTITUTION: An octapeptide derivative of the formula PG-A3-A4-A5-A5-Phe- Phe-Arg-Leu-FlG [PG shows an amino group-protecting group; A3, A4, A5 and A6 are each an amino acid shown by Ala, Asp, Lys, Leu, Arg, Ser or Pro; FlG shows a fluorescent group such as 4-methylcoumaryl-7-amide (MCA)]. This method for measuring proteinase A activity and foam stability of beer uses a nanapeptide derivative of the formula fg-A3-A4-A5-A6-Phe-Phe-Arg-Leu-Lys(Dnp)-NH2 [fg is a fluorescent group of (7-methoxycoumarin-4-yl)acetyl (MOCAc) group or anthraniloylbenzyl (ABz) group; A3, A4, A5 and A6 are each independently an amino acid shown by Ala, Asp, Lys, Leu, Arg, Ser or Pro; (Dnp) is dinitrophenyl group].;COPYRIGHT: (C)1996,JPO
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机译:目的:提供一种用于测定/测量啤酒中极少量蛋白酶A的方法,并获得可用于该方法的底物。 ;组成:式PG-A 3 Sub> -A 4 Sub> -A 5 Sub> -A 5 Sub>的八肽衍生物-Phe-Phe-Arg-Leu-FlG [PG表示氨基保护基; A 3 Sub>,A 4 Sub>,A 5 Sub>和A 6 Sub>是Ala,Asp, Lys,Leu,Arg,Ser或Pro;图1G显示了荧光基团,例如4-甲基香豆基-7-酰胺(MCA)。这种测定啤酒蛋白酶A活性和泡沫稳定性的方法是使用分子式为fg-A 3 Sub> -A 4 Sub> -A 5 Sub>的纳肽衍生物-A 6 Sub> -Phe-Phe-Arg-Leu-Lys(Dnp)-NH 2 Sub> [fg是(7-甲氧基香豆素-4-基)乙酰基的荧光基团(MOCAc)基团或蒽基苄基(ABz)基团; A 3 Sub>,A 4 Sub>,A 5 Sub>和A 6 Sub>分别独立地是Ala,Asp所示的氨基酸,Lys,Leu,Arg,Ser或Pro; (Dnp)是二硝基苯基] 。;版权:(C)1996,日本特许厅
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