STABILIZATION OF PROTOPLAST AND ARTIFICIAL CELL WALL-COATED PROTOPLAST

摘要

PURPOSE: To stabilize protoplast useful in the cell technology and biotechnology, etc., by culturing protoplast in a hydrophobicized polysaccharide-contg. medium to coat its cell membrane with the hydrophobicized polysaccharide. ;CONSTITUTION: The protoplast of vegetable cells (pref. carrot protoplast) is cultured in a medium containing a hydrophobicized polysaccharide (pref. partially hydrophobicized pullulan, amylose, mannan, amylopectin, dextran or xylogl-ucan phosphate) to coat its cell membrane with the polysaccharide to temporarily form artificial cell wall, thus stabilizing the protoplast.;COPYRIGHT: (C)1996,JPO