Cleaning agent is used for cleaning fabrics, acts on behalf of for washing dish, detergent, the fabric of cleaning method, method of washing Hot Dishes washing process

摘要

The present invention relates to a kind of cleaning compositions containing protease,This protease is that have in nature not it has been found that a kind of carbonylic hydrolase mutation for the amino acid sequence crossed,It is to be derived and with the more amino acid in different amino acid replacement precursor carbonyl hydrolases,The described more amino acid being wherein replaced in preemzyme is corresponding to position+76 in conjunction with one or more following resi-dues :+99,+101,+103,+104,+107,+123,+27,+105,+109,+126,+128,+135,+156,+166,+195,+197,+204,+206,+210,+216,+217,+218,+222,+260,+ 265 and/or+274. Here the position that number is indicated is corresponding to the position in the naturally occurring subtilisin obtained by bacillus amyloliquefaciens (Bacillus amyloliquefaciens) or corresponding to the position of amino acid residue of equal value in other carbonylic hydrolases or subtilisin (such as bacillus lentus (Bacillus lentus) subtilisin).