method for producing preferably suitable for detergents and cleaning agents, inhibitorfreien, easy wasserloeslichen enzymes, as well as those containing washing and cleaning agents, and processes for the manufacture of washing and cleaning agents

摘要

1283477 Trypsin; pancreatin COLGATEPALMOLIVE CO 6 March 1970 [19 March 1969] 10871/70 Heading C3H [Also in Division C5] Trypsin is prepared by: (i) comminuting freshfrozen animal pancreas glands; (ii) extracting with a C 1 or C 2 alcohol (methanol or ethanol per se or in aqueous solution); (iii) treating the liquid extract with a water-immiscible solvent (e.g. n-butanol or diethyl ether); (iv) precipitating the trypsin from the liquid with a C 1 or C 2 alcohol or a water-soluble ketone (e.g. acetone) at below 10‹ C.; and (v) recovering the enzyme (e.g. by filtration). The residue from (ii) may be extracted with acetone and dried, to give pancreatin. The trypsin may be incorporated in detergent compositions.