ELECTRO-INSERTION OF PROTEINS INTO ANIMAL CELL MEMBRANES

摘要

The present invention concerns a method of incorporating a protein into a cell membrane comprising exposing a cell to an electric field while the cell is suspended in an electro-insertion medium in the presence of a buffered solution (suspension) of the protein(s) to be inserted, the protein bearing a hydrophobic membrane spanning sequence and then contacting the resultant cell with a resealing medium.