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The method for early diagnosis of large intestine adenoma malignization
The method for early diagnosis of large intestine adenoma malignization
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机译:大肠腺瘤恶性早期诊断方法
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摘要
The invention has relation to medicine, especially to oncology and immunology.he purpose of invention is improvement of the diagnosis of large intestine adenoma malignization.It is offered the method for diagnosis of large intestine adenoma malignization by means of biological material researches.The novelty consists of invenstigation of separated and clean lymphocites of the peripheral blood of sick for all that they divided by two samples, in the first are added the ram's erythrocites, the mixture is incubated at temperature 37°C during 15 min, and in the second sample - the serumless physiological medium and incubate it at temperature 4°C during 12±6 hours moreover in the first sample is carried out formation of rosette reaction and is determined percentage of rosettle formated cellsat the first day, and in the second sample - the cells wash out once more after cool preoncubation, the rosette formation reaction is carried out, the percentage of rosette formated cells are determinated after that the ratio of the second sample number to the first one and in the case of its value more 1,0 the adenomas malagnization is diagnosed.The method has sufficient sensibility and can be applied with the prime diagnosis purpose of the malagnizated adenomas of large intestine as well for sicks monitoring which are undergoed an treatment.
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