Extraction and purification of an anticoagulant principle from the south american leech, haementeria ghilianii

摘要

Applicant has isolated a Factor Xa inhibiting substance from the salivary gland extract of the leech H. ghilianii. The extract is subjected to separation using both DEAE-cellulose and heparin-agarose chromatography resins eluting with an increasing salt gradient. The extract is subjected to affinity chromatography using Factor Xa bound to an Affi-Gel-15 resin eluting with HEPES containing benzamidine. Reverse phase chromatography yields several purified peptides or proteins having Factor Xa inhibiting activity. The properties and clinical compositions of these FXa inhibitors are described.