Process for the expression of heterologous proteins fused to e. coli, use thereof, expression vectors and recombinant strains.

摘要

The present invention is relative to the field of biotechnology IN PARTICULAR THE USE OF DNA recombinant technology for the production of heterologous proteins. THE OBJECT TECHNICAL SHE IS develop a highly efficient method for expression of heterologous genes fused WAY "ESCHERICHIA COLI" which are encoded proteins which can be readily purified DUE TO THE FACT THAT THEY ARE synthesized in soluble form in the CELLULAR CYTOPLASM. TO ACHIEVE THIS, TO USE an expression vector, which contains a stabilizing sequence WHICH ONLY codifies for the first 58 Amino acids belonging to the N-terminus of human protein Interleukin-2, WHICH IS UNDER THE PROMOTER Tryptophan CURRENT "ESCHERICHIA COLI". This vector contains GENE IN ADDITION TO RESIST Ampicillin AS A MARK SELECTION AND TRANSCRIPTION TERMINATOR ION bacteriophage T4. IN PARTICULAR WHICH GENES ENCODING PROTEIN BY VIRUS antigenic HUMAN immunodeficiency (HIV 1 and 2) will be cloned into, obtaining high levels of expression of this protein TRANSFORMADOS lineages of bacteria "Escherichia coli". THE method, which is the object of the present invention may be employed for expression of heterologous proteins, recombined at high levels synthesized in insoluble form and Merged into "E. COLI", WHICH MAY BE USED IN THE PHARMACEUTICAL INDUSTRY PREPARATIONS FOR VACCINE DEVELOPMENT OR DIAGNOSTIC SYSTEMS IN THE FOOD INDUSTRY IN AGRICULTURE, ETC.