Detection of bacteria in blood culture bottles by time-resolved light scattering and absorption measurement

摘要

Methods and apparatus for detecting bacteria in samples using time- resolved light scattering and absorption measurement are disclosed. The present invention provides a system wherein a plurality of samples may be tested by providing a modulated excitation signal which is introduced into each sample and collected at a point disposed across each sample. The amplitude and phase of the collected signal are then processed to determine the time characteristics, modulation and phase of the signal after it has passed through the sample. By comparing time characteristics, modulation and phase with the time characteristics, modulation and phase collected at a previous point in time or alternatively from a sample known to be free of bacteria, the presence of bacteria may be determined, since it has been found that the presence of bacteria causes a significant change in the time characteristics, modulation and phase. Preferably, diode lasers are used to introduce modulated electromagnetic radiation into the samples and a multichannel plate photomultiplier tube is used to provide a signal from which the phase and amplitude can be determined. Alternatively, in other embodiments of the present invention the modulated electromagnetic radiation may be introduced using an optical switch and the multichannel plate photomultiplier tube may be replaced by avalanche photodiodes disposed against each sample that also create a signal containing time- averaged intensity, amplitude, and phase information.