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Process for massive conversion of clostridia into synchronized cells of critical length or refractile endospores
Process for massive conversion of clostridia into synchronized cells of critical length or refractile endospores
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机译:将梭菌大规模转化为临界长度或难降解内生孢子的同步细胞的过程
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摘要
A method is disclosed for producing specially prepared bacteria of the genus Clostridium for producing solvents, enzymes, antibiotics, toxic proteins, or spores. Cell elongation to a critical length of at least about 3x is induced in an economical, abundantly available growth medium by serial subculturing under controlled conditions to effect synchronization of growth in the number of the cells and their effective mass and to produce a substantially homogeneous cell population. At least about 0.01M of a divalent cation such as calcium is added to the synchronized cells of critical length to stabilize the cells against death, lysis or aggregation. Where bacterial production of solvents is desired, cell division is inhibited by temperature shift or by chemical means when the cells reach a synchronized solventogenic state. Solvents produced by the specially prepared bacteria may be economically and readily recovered by conventional distillation procedures or the like. If the specially prepared bacteria are to be used for production of products other than solvents, growth of the synchronized, stabilized cells is inhibited by temperature shift and the cells are allowed to differentiate and continue metabolism and sporulation until the resultant bacteria are useful for preferential production of the desired enzymes, antibiotics or toxic proteins. Spore formation may be permitted to continue until substantially the entire cell population constitutes refractile mature free spores, which may be harvested and stored for future use.
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