CONJUGATES OF HEPARIN-BINDING EPIDERMAL GROWTH FACTOR-LIKE GROWTH FACTOR WITH TARGETED AGENTS

摘要

Conjugates of heparin-binding epidermal growth factor-like growth factor (HBEGF) linked, either directly or via a linker, to a targeted agent are provided. The targeted agent is a cytotoxic agent, such as a ribosome-inactivating protein (RIP) and an antisense nucleic acid, or is a therapeutic nucleic acid for targeted delivery. The targeted agent is attached to HBEGF, or via a linker, through a chemical bond, or the conjugate is prepared as a chimera using techniques of recombinant DNA. The conjugates are used to target cytotoxic agents or therapeutic nucleotides to cells bearing EGF receptors and are particularly useful for treating solid tumors, such as breast and bladder tumors, and for treating disorders involving pathophysiological proliferation of smooth muscle cells, such as restenosis.