A METHOD FOR PREPARING NON-PROTEINACEOUS STRONGLY NEGATIVELY CHARGED MACROBIOMOLECULES, WHICH ARE LINKED TO PLASTIC

摘要

The subject invention is directed at a method for preparing non-proteinaceous macrobiomolecules with a strong negative charge linked to plastic. Strong negatively charged biological macromolecules like desoxyribonucleic acid (DNA), ribonucleic acid (RNA) and glycosaminoglycans (GAG) play a crucial part in cell functioning. In principle, strongly negative charge implies at least one negative charge per pentasaccharide of the biomolecule in so far as it is composed of pentasaccharide. DNA and RNA contain one negative charge per nucleotide. Glycoso amino glycans are heterogeneous in this respect and can contain 1 to 4 negative charges per disaccharide. The invention is also directed at the product of the method, in particular a microtitre plate with plastic wells coated with negatively charged non-proteinaceous macrobiomolecules.