Antithrombin-III is purified by contacting an aqueous solution of antithrombin-III derived from human plasma with an insoluble carrier containing as a ligand a hydrophobic group and recovering a non-adsorbed fraction. By this purification, there are provided antithrombin-III preparations containing human plasma-derived antithrombin-III, in which virus is inactivated and which are substantially free from contaminants of at least pyrogen and thermally denatured protein produced by the inactivation.
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