Hapten conjugates with bioluminescent label for use in competitive immunoassays

摘要

Process for detecting an analyte in a liq sample by competitive immunoassay comprises: (a) incubating the liq. sample with a conjugate (A) and a receptor (B) in the presence of a reactive solid phase, where (A) is a conjugate of 1 hapten (I) that is immunologically concurrent with the analyte and a Ca-activatable photo-protein (II), so that the hapten is coupled to an amino gp. of the photo-protein, and the receptor is bonded to the solid phase or is able to bind to the solid phase, and can undergo a specific immunological reaction with the analyte and the hapten; (b) sepg. the solid phase from the incubation fluid, and (c) detecting the presence or the amt. of the analyte in the sample by luminescent measurement in the solid phase and/or the incubation fluid. Also claimed is a reagent (R) for competitive immunoassay of an analyte, comprising a conjugate of 1 (I) and (II) with (I) coupled to an amino gp. of (II); a reagent kit comprising (R) and, separately, (B) which is bound to a solid phase or able to bind with a solid phase; and a conjugate (C) comprising 1 hapten and (II), where the hapten is bonded to an amino gp. of (II) by a spacer with a chain length of 2-8 atoms.