NUCLEOTIDE SEQUENCE ENCODING CARBAMOYL PHOSPHATE SYNTHETASE II having genetic information of carbamoyl phosphate synthase II

摘要

According to the present invention, the genetic information of the carbamoyl phosphate synthase II of plasmodiumphalisporum, which is an enzyme involved in the first step reaction that determines the rate of the overall reaction of de-novo biosynthesis of pyrimidine, A nucleic acid molecule which is encoded and retained is provided. blood. Because it is not available to regenerate pyrimidine, it depends only on the pyrimidine produced by the de-novo biosynthetic process. On the other hand, mature loving red blood cells are so small that the demand for pyrimidine nucleotides is negligible. Thus, this enzyme becomes the first target site for the chemotherapeutic agent to function. The present invention relates to a method for producing carbamoyl phosphate synthetase II by a recombinant technique using a nucleic acid sequence encoding carbamoyl phosphate synthase II, and also relates to a method for producing carbamoyl phosphate synthetase II using complementary molecules, ribozymes, And to other gene inactivating drugs thereof.