Production method of Newcastle disease vaccine using animal cell culture method

摘要

The present invention provides a method for producing Newcastle disease vaccine through animal cell culture. The production method of the present invention comprises the steps of (a) obtaining an animal cell culture by culturing the animal cells in a jackjack orientation medium; Inoculating and adsorbing Newcastle disease virus on animal cell culture and then culturing; And (c) recovering the virus infection solution into the culture broth obtained in the previous step, wherein the animal cells usable in the present invention are fibroblasts or vero cell lines of 10-day-old fetuses, and these animal cells Can be cultured using a liquid culture or using a surface-action carrier or a porous carrier. In addition, according to the present invention, the vaccine can be stably produced at a high concentration by adding 0.5-10.0 mM EDTA after adsorbing the virus in step (b).