首页>
外国专利>
Method for producing human alpha interferon by fermentation of recombinant Escherichia coli with feed-batch ash
Method for producing human alpha interferon by fermentation of recombinant Escherichia coli with feed-batch ash
展开▼
机译:饲料灰分发酵重组大肠杆菌生产人α干扰素的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to a method for mass production of human alpha interferon by culturing a recombinant Escherichia coli containing an inducible promoter using the L-arabinose operon of Salmonell strain in a medium containing glycerol and yeast extract as a basic component Arabinose was added at a predetermined time to induce the induction of the expression, and then the amount of oxygen in the culture medium was supplied as a nutrient index of the depleted component, thereby continuously inducing the expression of the recombinant gene together with the growth of the cells, Alpha interferon is accumulated in cells as an inclusion body.
展开▼