METHOD OF PREPARING CURATIVE IMMUNOGLOBULINS AGAINST PLAGUE ('IMMUNOTCHUM'), PARVOVIRAL ENTERITIS ('IMMUNOPARV') AND ADENOVIRAL HEPATITIS ('IMMUNOHEPS') IN CARNIVOROUS

摘要

FIELD: veterinary virology, immunology. SUBSTANCE: method involves animal-donor immunization (sheep, goats) with cultural viral antigen - subcutaneously firstly with incomplete and/or complete Freund's adjuvants and then - without Freund's adjuvant at a single dose at all immunization steps (10: 25) at antigen titer 1g LD, not less. Patient blood samples were taken periodically at blood antibody titer 2.5 1g, not less, in reaction of biological neutralization for plague viruses and adenoviral hepatitis in carnivorous and less 1:512 in hemagglutination reaction inhibition for parvoviral enteritis. Animal-donors were revaccinated periodically with antigen dose of the same titer without Freund's adjuvant. Immunoglobulins were isolated by salt precipitation followed by purification by preparative chromatography. Invention can be used for preparing curative-prophylaxis immunoglobulins. EFFECT: improved method of immunoglobulin preparing.