METHOD OF PREPARING ANTIGEN FOR DIAGNOSIS OF SALMONELLA TYPHIMURIUM INFECTION IN POULTRY

摘要

FIELD: veterinary science, microbiology. SUBSTANCE: method involves preparing bacterial mass of S. typhimurium. O-antigenic fraction is isolated from bacterial mass and used it for sensibilization of formalized ram erythrocytes. Alternative method: O-antigenic fraction is isolated by an aqueous extraction of bacterial mass at 95-100 C for 2-2.5 hr followed by its fractionating with 6-6.5 ethanol volumes. EFFECT: enhanced sensitivity and specificity of antigen.