Production of quercetin glucuronide and this connection cells containing.

摘要

PURPOSE:To make quercetin glucuronide to be safely and widely utilized by extracting a specific cultured cell. CONSTITUTION:A leaf of Euphorbia millii as a plant belonging to genus Euphorbia is washed with deionized water and extracted by alcohol, etc., then cut, thus the resultant small piece is cultured in a medium containing auxin-affecting substance, etc., by light irradiation to obtain cultured cell (B) as callus, differentiated tissue, cultured organ, cultured root or cultured foliage abundantly containing quercetin glucuronide (A). Next, the component B is freeze-dried and extracted with a neutral organic solvent (C) such as butanol. to obtain an extracted solution (D). Then, the component D is filtered and resultant filtered solution is concentrated under reduced pressure below 40 deg.C, then washed with ether, etc., thus extracted with butanol, etc., and separated with liquid chromatography, etc., then purified to produce the aimed quercetin glucuronide.