首页> 外国专利> Thermostable arabino furanoside produced by Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661

Thermostable arabino furanoside produced by Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661

机译:嗜热脂肪芽孢杆菌NRRL B-18659,嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661生产的热稳定阿拉伯糖呋喃糖苷

摘要

An isolated arabinofuranosidase from Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 is disclosed. The arabinofuranosidase has a maximum activity at about pH 6.0 and at about 65° C., maintains at least about 50% of its maximum activity at 70° C. and pH 7.0 after 80 minutes, and has an isoelectric point of about 4.4. The arabinfuranosidase can be used in a method of hydrolyzing xylan present in wood pulp at temperatures of at least about 60° C. and a pH of at least about 7.0. The arabinofuranosidase is used along with at least two xylanases and a xylosidase isolated from the above Bacillus stearothermophilus strains.
机译:公开了从嗜热脂肪芽孢杆菌NRRL B-18659,嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661分离的阿拉伯呋喃糖苷酶。阿拉伯呋喃糖苷酶在约pH 6.0和约65℃下具有最大活性,在80分钟后在70℃和pH 7.0下保持其最大活性的至少约50%,并且具有约4.4的等电点。阿拉伯呋喃糖苷酶可用于在至少约60℃的温度和至少约7.0的pH下水解木浆中存在的木聚糖的方法。阿拉伯呋喃糖苷酶与至少两种木聚糖酶和木糖苷酶一起使用,这些木聚糖酶和木糖苷酶是从上述嗜热脂肪芽孢杆菌菌株中分离出来的。

著录项

  • 公开/公告号US5491087A

    专利类型

  • 公开/公告日1996-02-13

    原文格式PDF

  • 申请/专利权人 NOVO NORDISK A/S;

    申请/专利号US19950418044

  • 发明设计人 DANA D. ELM;BRUCE L. ZAMOST;

    申请日1995-04-06

  • 分类号C12N9/24;C12N1/20;C12N1/00;D21C3/00;

  • 国家 US

  • 入库时间 2022-08-22 03:39:02

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