Production of binding sites for PAF, PAF analogues and PAF antagonists in endothelial cells

摘要

The invention refers to the production of substances which prevent and treat disorders caused by pathological cell differentiation leading to enhanced LA-paf effect and production. The invention refers to simple methods to test and produce LA-paf antagonists and paf antagonists against very high affinity paf receptors on endothelial cells. According to the invention genes producing endothelial paf receptors in endothelial cells during differentiation for example in response to insulin can be transferred from adherent endothelial cell lines to soluble cells. Endothelial cell lines for example immortalized endothelial cells can also be cultured on soluble particles for screening procedures. Substances which prevent and inhibit very high affinity paf receptors and LA-paf production antagonize pathological differentiation, irritation, aging and death of cells. The antagonists can be administered for example with food.