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In-situ hybridization probes for identification and banding of specific human chromosomes and regions
In-situ hybridization probes for identification and banding of specific human chromosomes and regions
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机译:原位杂交探针,用于鉴定和标记特定的人类染色体和区域
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摘要
The invention relates to novel primer sets useful in preparing DNA probes specific for any chromosome or part of a chromosome, particularly human chromosomes. The DNA probes so produced may be used to paint individual chromosomes or portions of chromosomes in metephase cell spreads and in interphase nuclei. When used to paint chromosomes in metephase spreads, R-bands are readily detectable. The method is sensitive and has been shown to paint R-bands on chromosomes pieces having as few as several hundred kilobases.
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