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Cloning and expression of a gene encoding bryodin 1 from Bryonia dioica
Cloning and expression of a gene encoding bryodin 1 from Bryonia dioica
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机译:圆白菜(Bryonia dioica)bryodin 1编码基因的克隆与表达
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摘要
The molecular cloning and expression of biologically active ribosome- inactivating protein bryodin 1 are described. A complete amino acid and oligonucleotide sequence encoding bryodin 1 are also described. Further, plasmids, expression vectors comprising a nucleotide sequence encoding bryodin 1 and transformed host cells are described. Isolation and characterization of the nucleotide sequence for bryodin 1 enables the recombinant production of large amount of bryodin 1 for use in vitro or in vivo directly or as ligand/toxin conjugates or fusion proteins. These compositions can be used to selectively kill undesired cells such as cancer cells, infected cells, bacteria.
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