Methods for preparing polyacrylamide gels for electrophoretic analysis

摘要

This invention relates to a method and system for producing a polyacrylamide gel sample typically for use in electrophoretic analysis. The subject method includes providing a container comprising a bottle and cap assembly for conducting a gel formation sequence. The cap assembly includes a storage compartment. A plurality of premixed chemical materials are employed for producing a polyacrylamide gel used to make the gel samples. The container includes a bottle for storing an acrylamide solution and a cap assembly for storing the premixed chemical materials in dry particulate form therewithin. The cap is located atop the bottle. The premixed chemical materials are stored in the bottle and in the storage compartment for subsequently producing the acrylamide gel solution in situ within the confines of the container so that the step of transferring of the gel stock solutions to an auxiliary container is avoided. A polyacrylamide gel sample for use in electrophoretic analysis can be produced from the above-described acrylamide gel solution. The sample is produced within a leak-proof formation chamber typically includes a pair of plates and a easily-alignable, unitary, substantially U-shaped spacer member.