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Lead detection method and reggents utilizing aminolevulinic acid dehydratase and tertiary phosphines
Lead detection method and reggents utilizing aminolevulinic acid dehydratase and tertiary phosphines
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机译:利用氨基乙酰丙酸脱水酶和叔膦的铅检测方法和试剂
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摘要
A method and kit for simplifying and improving the sensitivity and accuracy of a lead assay for a sample solution suspected of containing lead determines the extent of a reaction between a substrate and a disulfide enzyme in the presence of an activating reagent which contains a water-soluble tertiary phosphine reagent so as to increase the activity of the disulfide enzyme for reaction with the substrate. For a colorimetric determination of the enzyme activity a chromophore is formed upon reaction with a selected component of the sample solution in the presence of a colorimetric enhancing reagent. The colorimetric enhancing reagent contains a metal ion such as cupric ion or ferric ion which is soluble in the sample solution. The extent of the chromophore formation is then photometrically determined.
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