Positive / negative cell selection and positive cell selection is mediated by peptide release

摘要

(57) for discharging cells are positively selected by binding to a solid support or other beads via an antibody from a heterogeneous cell suspension [Abstract] The present invention relates to a method for non-enzymatic I provide. Involves the formation of a complex containing the cell suspension in solid supports coupled to the primary monoclonal antibody, which is, the method is bound to the cell surface antigens of the target cells are then. Were separated from the cell suspension and the complex then is contacted with a specific peptide which binds to the primary antibody was replaced with cell surface antigens and antibodies, the results, it is released from the complex to target cells. Further, the present invention provides a method for the positive / negative cell selection. Here, the target cell having a first antigen is selected from a heterogeneous cell suspension containing cells that are not desired with a second antigen. The present invention provides a method for identifying a specific peptide useful for releasing a target cell from the binding of specific monoclonal antibodies. The methods of the invention are particularly useful for the purification of lymphocytes or tumor cells is not desirable from a cell population positively selected and the accompanying positive selection of CD34 + hematopoietic stem cells. Then, CD34 + cells purified composition is useful for for reconstructing the immune system of the patient, the reinfusion of cancer patients after treatment of high capacity.