METHOD FOR ISOLATING PROTEIN FROM WASTE CULTURE SOLUTION AFTER SHORT PERIOD CULTURE OF BCG BACTERIA AND MEASURING REAGENT FOR DELAYED HYPERSENSITIVITY REACTION

摘要

PROBLEM TO BE SOLVED: To provide a method for efficiently recover a useful protein excreted by BCG bacteria in a culture medium and provide the recovered protein as a reagent, by using a waste culture medium from a short period culture of the BCG bacteria which is discharged in large amount as an industrial waste. ;SOLUTION: The subject protein is obtained by heating a waste culture medium at 40-45°C, successively filtering BCG bacteria cells and small molecular weight culture medium components to obtain a concentrated protein and carrying out an affinity purification of the protein successively by a phenylsepharose CL-4B column, a DEAE-sepharose column using urea, a sephacryl S200HR column and finally a DEAE-sepharose CL-6B column without using urea. The protein is used as a reagent.;COPYRIGHT: (C)1997,JPO