Homogenate eggplant immunoassay utilizing a mutation glucose-6-phosphate dehydrogenase

摘要

(57) [summary] The present invention relates to a method for the immunoassay of an analyte that utilize mutation glucose-6-phosphate dehydrogenase (G6PDH) enzyme as a label. For more information, deletion of one or more amino acids per subunit, insertion or substitution, or any present invention is the precursor G6PDH and any analyte analogue or analyte, and a mutation NAD ^+-dependent G6PDH on the use of enzymes which differ by a combination of them. The present invention also encompasses the construction of multiple mutations in the precursor of glucose-6-phosphate dehydrogenase (G6PDH) enzyme also. In general, the introduction or deletion of a lysine residue of one or more, or the introduction of cysteine ​​residues of one or more due to the insertion of a cysteine ​​to precursor G6PDH, or this mutation comprehensive in the substitution of cysteine ​​precursor G6PDH amino acid residues to. DNA sequence encoding the enzyme cell lines, the task of producing the enzyme kits useful, the problem to implement a conjugate of a member of the specific binding pair with an enzyme of the challenges, the method of the present invention The present invention , as well as a vector and a DNA encoding the enzyme of the task was designed host cells to allow the production of enzymes and challenges.