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GLUCOSIDE DERIVATIVE FOR ENZYME MODIFICATION, ORGANIC SOLVENT-SOLUBLE ENZYME MODIFIED WITH THE SAME AND ITS PRODUCTION
GLUCOSIDE DERIVATIVE FOR ENZYME MODIFICATION, ORGANIC SOLVENT-SOLUBLE ENZYME MODIFIED WITH THE SAME AND ITS PRODUCTION
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机译:用于酶改性的糖苷衍生物,经其改性的有机溶剂可溶酶及其生产
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摘要
PROBLEM TO BE SOLVED: To obtain a glucoxide derivative which is inexpensive and readily available, has a specific chemical structure, modifies an enzyme to impart the the enzyme solubility in organic solvent as well as sustain its high enzymatic activity in the organic solvent. ;SOLUTION: This glucoxide derivative has a chemical structure of' the formula (R is a 6-22C hydrocarbon group), for example, N,N-dilauryl-D-gluconic acid amide. This derivative is obtained, for example, by dissolving (A) a secondary amine disubstituted with a 6-20 hydrocarbon group and (B) glucono-1,5-lactone in a solvent boiling at 60-250°C such as methanol and allowing them to react with each other at the refluxing temperature. When an enzyme is modified with this compound, the weight ratio of this compound to the enzyme is 0.2-100.;COPYRIGHT: (C)1997,JPO
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