P. Cloning and expression of gene II and chondroitinase I from vulgaris

摘要

(57) [summary] The present invention encodes a major protein component of chondroitinase ABC called Proteus vulgaris that is included in the Nsi fragment shown in Figure 1 from (P. vulgaris) and "chondroitinase I" on the DNA sequence. The present invention, P. Cloning and expression of a gene containing DNA sequences encoding the protein component of the second of chondroitinase ABC called from vulgaris as "chondroitinase II", these DNA sequences, amino acids and II recombinant chondroitinase I array, and isolation process for purifying II or recombinant chondroitinase I. These methods, P. Over methods obtained by modifying the recombinant enzyme for the method used previously to isolate and purify chondroitinase I from natural vulgaris, to provide high yield and purity quite. See Figure 1.