IMMUNOLOGICAL AGGLUTINATION REAGENT AND IMMUNITY ANALYSIS METHOD

摘要

PROBLEM TO BE SOLVED: To provide an immunization agglutination reagent for measuring the amount of agglutination having sufficiently high sensitivity in practical use and physicochemical stability, which is not found in conventional liposome agglutination reagent and an immunity analysis method using the immunization agglutination reagent. ;SOLUTION: As the carrier of immunological agglutination reagent, the liposome, on which osmotic pressure is loaded by the substitution of the external water phase of the liposome with the aqueous solution having the osmotic pressure higher than the osmotic pressure of the hydration aqueous solution when the liposome is prepared by 120mOsm or more, is used. In details, the osmotic pressure of the hydration aqueous solution is made to be 0.5-30mOsm, the first substitution is performed so that the difference of the osmotic pressures of the hydration aqueous solution and the first substituted aqueous solution becomes 320mOsm or more and the small particle diameter and the high turbidity of the liposome are achieved. When the undesirable effect is observed after the first substitution, it is recommendable that the second substitution is performed with the second substitution aqueous solution having a osmotic pressure of 700mOsm or less.;COPYRIGHT: (C)1997,JPO