FRUCTOSYLAMINO ACID OXIDASE AND ITS PRODUCTION

摘要

PURPOSE: To obtain the subject enzyme useful for diagnosing diabetic symptoms, administering such symptoms, and understanding the food preservation status and/or period after producing foods, through the quantitative analyses of Amadori compounds. ;CONSTITUTION: This enzyme is obtained by culturing in a fructosyllysine-contg. medium Penicillium bacteria capable of producing fructosylamino acid oxidase [e.g. Penicillium janthinellums-3413 (FERM BP-5475)] and has the following physicochemical properties: (1) catalyzing the reaction forming an α-ketaldehyde, amine derivative and H₂O₂ by oxidizing an Amadori compound in the presence of oxygen; (2) optimum pH : 7.5, and s table pH: 4.0-11.0; (3) optimum temperature: 25°C, and stable temperature: 15-50°C; and (4) molecular weight: about 38700 (gel filtration method).;COPYRIGHT: (C)1996,JPO