Immunity histochemistry assaying modulo null in order to detect tumor join marker p53

摘要

This invention is an immunohistochemical p53 assay method in which human tissue sections or cells are fixed on a glass slide and incubated with anti-p53 antibodies which react with the p53 antigen in the specimen. The p53 antigen-antibody complex is then localized by addition of anit-lg which is either labeled directly or indirectly with an enzyme. Next, a chromogen substrate solution for the enzyme is added to the slide and color is developed. The slide is then examined under a microscope for specifically stained cells which indicate the presence of p53 antigen.