Nutrient medium null for central neurocyte

摘要

PURPOSE: To obtain the subject new medium capable of achieving continuously stable adhesion of nerve cell, extension of projection of nerve and survival maintenance without requiring coating of cell adherent protein, comprising a cultured supernatant liquid of established hepatocyte prepared by serum-free culture as an active ingredient. ;CONSTITUTION: The objective medium comprising a cultured supernatant liquid of established prepared by serum-free culture as an active ingredient. To be concrete, a hepatocyte such as NTCC clone 1,469 is cultured in 10% bovine fatal serum-containing DME/F-12 medium until the hepatocyte becomes semiconfluent, the medium is changed into a serum-free medium from which serum is removed, the hepatocyte is cultured for 2-3 days and the cultured supernatant liquid is used as an active ingredient for a medium for central neurocyte.;COPYRIGHT: (C)1993,JPO&Japio