Fimbrias fimbrial and process for the preparation of fimbrias fimbrial

摘要

Fimbriae adhesins have a molecular weight of 2x105 and 2x107 Da, and comprise 90-95% protein and 1-3% sugar. Type 1 fimbriae show 5 different protein fractions of 14-20 kDa, most of which are associated to carbohydates. Type P fimbriae also include 5 different protein fractions of 14-20 kDa, one of the majority ones being associated to carbohydrates. The process comprises: culturing E. coli strains CECT 4484 and CECT 4485; collecting the sediment by centrifugation, resuspending it in physiological saline and homogenizing it; centrifuging the homogenate collecting the supernatant, which is subjected to saline precipitation, reconstituting the precipitate and dialyzing the solution; treating the dialyzate with sodium deoxcholate and, subjecting the product to two successive chromatographies with Sephacryl S-200 and Sepharose 4B. Use in treating and preventing infections of the urinary tract produced by fimbriated E. coli.