Pesticide compositions containing protein, Bacillus strains, proteins and genes that encode Proteins of Bacillus.

摘要

The present Invention relates to compositions and Methods for controlling plant pests.In particular pestici - das provided new Proteins that are produced during the ve getative Growth strains of Bacillus and P - nas such agents are useful as pesticides.More specific cificamente interacts with a Strain of Bacillus produces a substantially Purified prote ina Pesticide during Vegetative Growth, where the Bacillus is not B.Sphaericus SSII - 1.It is a specific protein in areas that can be isolated during the Vegetative phase of cre Foundation,Where this Protein is pre ferred toxic to insects of the orders Lepidoptera and Coleoptera or presents a Mole - cular weight around 30kda or more.This Protein has a Spectrum of activity that includes insecticidal activity against Spodoptera species Agrotis and / or.This Protein is capable of Isolating Insect, for example, Bacillus cereus with registration number NRRL B - 21060 and others.Another Preferred embodiment of the invention sets an Auxiliary Protein com - Where the sequences that represent the signal secretion have been removed or Inactivated Proteins Pesti - CI;Das multimeric over a Chain polipep - tidica can Activate or enhance Pesticide Insect specific activity; In addition,Fusion Proteins comprising several Protein Domains Invention produced by genetic FUSIONS is Preferred; especially a fusion protein comprising a Protein is- Insect specifies as indicated in SEC ID no. 5 and an Auxiliary Protein as indicated in SEC ID no. 2 that leads to Protein as indicated in SEC ID no.23, including any Protein that is a structural and / or functionally homologous to it.Another aspect relates to a DNA Molecule comprising a Nucleotide sequence that encodes a Protein of Insect specific object of this Invention.Also an expression cassette comprising a DNA Molecule of the invention together with a sane Way of expression in operable to sequences that include the signals regulating the transcripciOn Translation were necessary and associated with a host Agency.